采用优化的数字PCR方法分析转基因小麦外源基因拷贝数
琚鹏举1,宁蕾1,葛林豪2,许成杰1,史华伟1,梁凯歌3,马亮4,刘陶然2,陈明2(
),孙黛珍1()
),孙黛珍1()
Analysis of Foreign Gene Copy Number in Transgenic Wheat by Optimized Digital PCR
JU PengJu1,NING Lei1,GE LinHao2,XU ChengJie1,SHI HuaWei1,LIANG KaiGe3,MA Liang4,LIU TaoRan2,CHEN Ming2(),SUN DaiZhen1()
),SUN DaiZhen1()
图8. 在nib8不同位置设计引物的结果图（第12号转nib8株系）
A和B分别是用nib8不同区间引物对第12号转nib8株系内参基因和目标基因的数字PCR检测;C：第12号转nib8株系的拷贝数检测
Fig.8. Results of designing primers at different positions of nib8 gene (take 12 strains as an example)
A and B were used to detect the internal reference gene and target gene of the no.12 transferred nib8 line by digital PCR with primers of different regions of nib8; C: Copy number detection of No. 12 to nib8 line
