采用优化的数字PCR方法分析转基因小麦外源基因拷贝数
琚鹏举1,宁蕾1,葛林豪2,许成杰1,史华伟1,梁凯歌3,马亮4,刘陶然2,陈明2(
),孙黛珍1()
),孙黛珍1()
Analysis of Foreign Gene Copy Number in Transgenic Wheat by Optimized Digital PCR
JU PengJu1,NING Lei1,GE LinHao2,XU ChengJie1,SHI HuaWei1,LIANG KaiGe3,MA Liang4,LIU TaoRan2,CHEN Ming2(),SUN DaiZhen1()
),SUN DaiZhen1()
图7. 转nib8株系的拷贝数分析
A1和B1分别以Wx012和SSII为内参的转nib8第12号株系的拷贝数分析;C1和D1分别以Wx012和SSII为内参的转nib8第17号株系的拷贝数分析;E1和F1是阴性对照和空白对照
Fig.7. Copy number analysis of transferred nib8 lines
Copy number analysis of transferred nib8 line no. 12 with Wx012 and SSII as internal parameters for A1 and B1; The copy number analysis of C1 and D1 with Wx012 and SSII as internal parameters, respectively; E1 and F1 are negative controls and blank controls
