采用优化的数字PCR方法分析转基因小麦外源基因拷贝数
琚鹏举1,宁蕾1,葛林豪2,许成杰1,史华伟1,梁凯歌3,马亮4,刘陶然2,陈明2(
),孙黛珍1()
),孙黛珍1()
Analysis of Foreign Gene Copy Number in Transgenic Wheat by Optimized Digital PCR
JU PengJu1,NING Lei1,GE LinHao2,XU ChengJie1,SHI HuaWei1,LIANG KaiGe3,MA Liang4,LIU TaoRan2,CHEN Ming2(),SUN DaiZhen1()
),SUN DaiZhen1()
图6. 内参基因的拷贝数分析
A1和B1是以PINb-D1b为内参基因时Wx012的拷贝数;C1和D1是以Wx012为内参基因时SSII的拷贝数;E1和F1是阴性对照和空白对照
Fig. 6. Copy number analysis of internal reference genes
A1 and B1 are the copy numbers of Wx012 when pinb-d1b is the internal reference gene.C1 and D1 are the copy numbers of SSII when Wx012 is the internal reference gene; E1 and F1 are negative controls and blank controls
