采用优化的数字PCR方法分析转基因小麦外源基因拷贝数
琚鹏举1,宁蕾1,葛林豪2,许成杰1,史华伟1,梁凯歌3,马亮4,刘陶然2,陈明2(
),孙黛珍1()
),孙黛珍1()
Analysis of Foreign Gene Copy Number in Transgenic Wheat by Optimized Digital PCR
JU PengJu1,NING Lei1,GE LinHao2,XU ChengJie1,SHI HuaWei1,LIANG KaiGe3,MA Liang4,LIU TaoRan2,CHEN Ming2(),SUN DaiZhen1()
),SUN DaiZhen1()
图3. 不同模板浓度条件下nib8（A）和内参基因（B）的数字PCR检测
蓝点为内参基因阳性,绿点为nib8阳性,灰点为阴性,红线为荧光阈值限,从左往右依次为20（A01）、40（B01）、160（C01）和320 ng（D01）模板及水
Fig. 3. Digital PCR results of nib8 gene(A) and control gene (B) at different template concentrations
The blue dots were positive for the internal reference gene, the green dots were positive for nib8, the gray dots were negative, and the red line was the fluorescence threshold. From left to right, the template and water were 20 (A01), 40 (B01), 160 (C01) and 320 ng (D01)
