采用优化的数字PCR方法分析转基因小麦外源基因拷贝数
琚鹏举1,宁蕾1,葛林豪2,许成杰1,史华伟1,梁凯歌3,马亮4,刘陶然2,陈明2(
),孙黛珍1()
),孙黛珍1()
Analysis of Foreign Gene Copy Number in Transgenic Wheat by Optimized Digital PCR
JU PengJu1,NING Lei1,GE LinHao2,XU ChengJie1,SHI HuaWei1,LIANG KaiGe3,MA Liang4,LIU TaoRan2,CHEN Ming2(),SUN DaiZhen1()
),SUN DaiZhen1()
图2. 不同退火温度条件下nib8（A）和内参基因（B）的数字PCR检测
蓝点为nib8阳性,绿点为内参基因阳性,灰点为阴性,红线为荧光阈值,从左往右依次为56℃、56.9℃、58.1℃、59℃、59.6℃、60℃、对照
Fig. 2. Digital PCR detection of nib8 (A) and internal reference genes (B) at different annealing temperatures
The blue point was positive for nib8, the green point was positive for internal reference gene, and the gray point was negative. The red line was the fluorescence threshold, which was 56℃, 56.9℃, 58.1℃, 59℃, 59.6℃, 60℃ and the control from left to right
