采用优化的数字PCR方法分析转基因小麦外源基因拷贝数
琚鹏举1,宁蕾1,葛林豪2,许成杰1,史华伟1,梁凯歌3,马亮4,刘陶然2,陈明2(
),孙黛珍1()
),孙黛珍1()
Analysis of Foreign Gene Copy Number in Transgenic Wheat by Optimized Digital PCR
JU PengJu1,NING Lei1,GE LinHao2,XU ChengJie1,SHI HuaWei1,LIANG KaiGe3,MA Liang4,LIU TaoRan2,CHEN Ming2(),SUN DaiZhen1()
),SUN DaiZhen1()
图1. 4对引物的特异性扩增
M：DL2000Maker;1：nib8;2：PINb-D1b;3：Wx012;4：SSII;5：H2O
Fig. 1. Specific amplification results of four pairs of primers
