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采用优化的数字PCR方法分析转基因小麦外源基因拷贝数
琚鹏举1,宁蕾1,葛林豪2,许成杰1,史华伟1,梁凯歌3,马亮4,刘陶然2,陈明2(),孙黛珍1()
Analysis of Foreign Gene Copy Number in Transgenic Wheat by Optimized Digital PCR
JU PengJu1,NING Lei1,GE LinHao2,XU ChengJie1,SHI HuaWei1,LIANG KaiGe3,MA Liang4,LIU TaoRan2,CHEN Ming2(),SUN DaiZhen1()

图1. 4对引物的特异性扩增
M：DL2000Maker;1：nib8;2：PINb-D1b;3：Wx012;4：SSII;5：H2O

Fig. 1. Specific amplification results of four pairs of primers