采用优化的数字PCR方法分析转基因小麦外源基因拷贝数

琚鹏举,宁蕾,葛林豪,许成杰,史华伟,梁凯歌,马亮,刘陶然,陈明,孙黛珍

Analysis of Foreign Gene Copy Number in Transgenic Wheat by Optimized Digital PCR

JU PengJu,NING Lei,GE LinHao,XU ChengJie,SHI HuaWei,LIANG KaiGe,MA Liang,LIU TaoRan,CHEN Ming,SUN DaiZhen

表4 探针法、染料法和数字PCR的结果对比

Table 4 Comparison between fluorescence quantitative method such as probe method and dye method, and digital PCR method

株系 Strain 染料法qPCR Dye method qPCR 探针法qPCR Probe method qPCR 数字PCR Digital PCR 12 7 8 7 16 1 1 1 17 1 1 1 23 1 1 1 29 1 1 1 30 6 7 7