采用优化的数字PCR方法分析转基因小麦外源基因拷贝数

琚鹏举,宁蕾,葛林豪,许成杰,史华伟,梁凯歌,马亮,刘陶然,陈明,孙黛珍

Analysis of Foreign Gene Copy Number in Transgenic Wheat by Optimized Digital PCR

JU PengJu,NING Lei,GE LinHao,XU ChengJie,SHI HuaWei,LIANG KaiGe,MA Liang,LIU TaoRan,CHEN Ming,SUN DaiZhen

表3 转nib8株系的数字PCR检测低限测定结果

Table 3 The results of low limit determination by digital PCR were obtained by transplanting nib8 strain

品系（基因） Lines (gene) 质量分数 Mass fraction (%) 用量 Dosage (ng) 检测拷贝数 Copy number (个/μL) 12 100 10 7 16 100 10 5 17 100 10 1 23 100 10 1 29 100 10 1 30 100 10 7