采用优化的数字PCR方法分析转基因小麦外源基因拷贝数

琚鹏举,宁蕾,葛林豪,许成杰,史华伟,梁凯歌,马亮,刘陶然,陈明,孙黛珍

Analysis of Foreign Gene Copy Number in Transgenic Wheat by Optimized Digital PCR

JU PengJu,NING Lei,GE LinHao,XU ChengJie,SHI HuaWei,LIANG KaiGe,MA Liang,LIU TaoRan,CHEN Ming,SUN DaiZhen

表1 数字PCR 探针引物序列

Table 1 Sequences of probes and primers used in digital PCR

序号No． 引物名称Primer name 引物序列Primer sequence (5'-3') 1 Nib8-F CTCAATGCTGCTATCACCATAGTG Nib8-R CACTCTTACT TGGCAGGAAT CTCA Nib8-P ATTGCAGCTAGGATGTCAGAGCCGTGTT 2 PINb-D1b-F AGTTGGCGGCTGGTACAATG PINb-D1b-R ACATCGCTCCATCACGTAATCC PINb-D1b-P TCTCAACAATGTCCGCAGGAGCGGCC 3 Wx012-F GGTCGCAGGAACAGAGGTGT Wx012-R GGTGTTCCTCCATTGCGAAA Wx012-P CAAGGCGGCCGAAATAGGTTGCC 4 SSII-F CACCATCAGTGAAGGAATGAATG SSII-R GGCGATATTTGGTACCTAATTGAAG SSII-P TACCCGATCGACCGTTTTGCC