番茄ShARPC5抗白粉病功能分析

冯婵婧,孙广正,王阳,马青

Functional Analysis of Gene ShARPC5 Involved in Tomato Resistance to Powdery Mildew

ChanJing FENG,GuangZheng SUN,Yang WANG,Qing MA

表1 qRT-PCR反应中的基因及引物

Table 1 Genes and primers in qRT-PCR reaction

基因 Gene 引物序列Primer sequence 正向引物Forward primer sequence (5′-3′) 反向引物Reverse primer sequence (5′-3′) ShARPC5/SlARPC5 CGAAGGCATAATCACAAGA CAGCAAGACAACGCAGTA PR1b1 CATCCCGAGCACAAAAC TGAAGTCACCACCACCCT Glucanase A CTTTTACTTGTTGGGCTTCT ACTTCCTTTGAGGGCATT Chitinase 3 ACGCCATCCCCTAAAGA TGGACCCATCCCACATT GAPDH (Control) CTGGTGCTGACTTCGTTGTTG GCTCTGGCTTGTATTCATTCTCG AtARPC5 CGGAATGCTCAATGCTCT CGGTCCCCAGTAGAAAGTC UBQ10 (Control) AGAAGTTCAATGTTTCGTTTCATGTAA TTACGAATCCGAGGGAGCCATTG