意大利蜜蜂工蜂中肠发育过程中的差异基因 表达谱及调控网络

杜宇,周丁丁,万洁琦,卢家轩,范小雪,范元婵,陈恒,熊翠玲,郑燕珍,付中民,徐国钧,陈大福,郭睿

Profiling and Regulation Network of Differentially Expressed Genes During the Development Process of Apis mellifera ligustica Worker’s Midgut

Yu DU,DingDing ZHOU,JieQi WAN,JiaXuan LU,XiaoXue FAN,YuanChan FAN,Heng CHEN,CuiLing XIONG,YanZhen ZHENG,ZhongMin FU,GuoJun XU,DaFu CHEN,Rui GUO

表1 本研究所用引物

Table 1 Primers used in this study

引物名称Primer name 引物序列Primer sequence XM_016912284.1-F AGCGATTGAAGATGTGCC XM_016912284.1-R ACGACAGGTGAAACCGAA TCONS_00023605-F GCAGTGACCTTGGCGTATT TCONS_00023605-R CATTCTGACCGTTGATTTGC XM_016912389.1-F TAAGGTGCTGCTACCACTGC XM_016912389.1-R GCTCTGTTAGGGTCGCATCT TCONS_00001615-F GTGGACGACACACGATTCT TCONS_00001615-R CCAATAACAGCGACACACG XM_001123053.4-F TTGATGCTGTGAAGGCTG XM_001123053.4-R AGGCGAACTGTAAACGGTT XM_016915022.1-F ATCAACAACACCCACACCT XM_016915022.1-R AGGAACGGATTCGCTGTA Actin-F CACTCCTGCTATGTATGTCGC Actin-R GGCAAAGCGTATCCTTCA ame-miR-6001-3p-loop CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGTAGTGGTA ame-miR-6001-3p-F ACACTCCAGCTGGGTTCTCTTTGGTTGT ame-miR-6001-3p-R CTCAACTGGTGTCGTGGAGTCG U6-F GTTAGGCTTTGACGATTTCG U6-R GGCATTTCTCCACCAGGTA