异源表达芥菜BjMATE增强紫花苜蓿耐酸铝胁迫的机理
李小冬1,2,尚以顺1(
),李世歌1,2,陈光吉1,2,裴成江1,2,孙方1,熊先勤1
),李世歌1,2,陈光吉1,2,裴成江1,2,孙方1,熊先勤1
The Mechanism of Ectopic Expression of Brassica juncea Multidrug and Toxic Compound Extrusion (BjMATE) to Enhance the Resistance to Acid and Aluminum Stress in Alfalfa
XiaoDong LI1,2,YiShun SHANG1(),ShiGe LI1,2,GuangJi CHEN1,2,ChengJiang PEI1,2,Fang SUN1,XianQin XIONG1
),ShiGe LI1,2,GuangJi CHEN1,2,ChengJiang PEI1,2,Fang SUN1,XianQin XIONG1
图1. 转基因植株的分子鉴定与基因表达分析
A：NPT和BjMATE引物检测转基因紫花苜蓿;B：NPT和BjMATE引物检测转基因拟南芥;C：转基因紫花苜蓿中BjMATE的qRT-PCR分析;D：转基因拟南芥中BjMATE的qRT-PCR分析。*表示在P<0.05水平差异显著
Fig. 1. Molecular detection and gene expression analysis of transgenic alfalfa and Arabidopsis
A: Transgenic alfalfa examination with NPT and BjMATE primers; B: Transgenic Arabidopsis examination with NPT and BjMATE primes. C: qRT-PCR analysis of BjMATE in transgenic alfalfa; D: qRT-PCR analysis of BjMATE in transgenic Arabidopsis. * indicate a significant difference were detected at P<0.05 level
