异源表达芥菜BjMATE增强紫花苜蓿耐酸铝胁迫的机理

李小冬,尚以顺,李世歌,陈光吉,裴成江,孙方,熊先勤

The Mechanism of Ectopic Expression of Brassica juncea Multidrug and Toxic Compound Extrusion (BjMATE) to Enhance the Resistance to Acid and Aluminum Stress in Alfalfa

XiaoDong LI,YiShun SHANG,ShiGe LI,GuangJi CHEN,ChengJiang PEI,Fang SUN,XianQin XIONG

表1 试验中用到的引物

Table 1 Primer used in the real time PCR assay

基因位点Gene locus 序列Sequence(5′-3′) 用途Purpose NPT F：GTGCCCTGAATGAACTGC 抗性基因PCR检测 R：CAATATCACGGGTAGCCA PCR with resistance gene primer 35S F：TCCCACTATCCTTCGCAAG 基因特异性引物检测 MATE R：TCAGACCGACGCATTTATCTTT Gene specific primer examination qBjMATE F：AAGGCGTTCTATCAGGAGTG BjMATE表达分析 R：GAGTGATGAAGATTGGGAAA qRT-PCR for BjMATE qAtMATE F：CTCTTGAGTTTCATGGGAGTA AtMATE表达分析 R：CGGGTCGAGTATTATGTTTG qRT-PCR for AtMATE qAtPIN2 F：CGAGTGGAGCAAGTGGAGTC AtPIN2表达分析 R：GTGGATACATCGGTGGAAGA qRT-PCR for AtPIN2 qAtALS3 F：AATGTTCTTGCTCGTCCTCC AtALS3表达分析 R：CTTGTCTTGGCGTTGCTCCT qRT-PCR for AtALS3 qAtALMT1 F：TCATTATTTGAGGAGCAGTC AtALMT1表达分析 R：AAAGAGTAACGCAAAGGAAA qRT-PCR for AtALMT1 qAtSTOP1 F：TTCATCAGACTGTGGGAATT AtSTOP1表达分析 R：AGGTTACTCAACATCGTCCT qRT-PCR for AtSTOP1 qMsGPDH F：ACAAACATGGGAGCATCCTTACTAG qRT-PCR（紫花苜蓿内参） R：GTTTTTACCGACAAGGACAAAGCT Reference gene in alfalfa qAtActin7 F：GATATTCAGCCACTTGTCTGTGAC qRT-PCR（拟南芥内参） R：CATGTTCGATTGGATACTTCAGAG Reference gene in Arabidopsis