黄瓜CsRPL1/2的克隆及其功能分析

宋维源,侯钰,赵剑宇,刘小凤,张小兰

Cloning and Functional Analysis of CsRPL1/2 in Cucumber

WeiYuan SONG,Yu HOU,JianYu ZHAO,XiaoFeng LIU,XiaoLan ZHANG

表1 引物序列

Table 1 Primers sequences

引物名称 Primer name 引物序列Primer sequence (5′-3′) CsRPL1-F ATGGCTGAGGGTATTGAATCCTAC CsRPL1-R TCAGCCCACAAAGTCATGTAACAGC CsRPL2-F ATGGCGGAGGGTTTTGAAGTTTAC CsRPL2-R TCAAAACCACTCATTTACAAACCCT CsRPL1-1300-F TACACCAAATCGACTCTAGAATGGCTGAGGGTATTGAATC CsRPL1-1300-A CCTTGCTCACCATGGTACCGCCCACAAAGTCATGTAACA CsRPL2-1300-F TACACCAAATCGACTCTAGAATGGCGGAGGGTTTTGAAGT CsRPL2-1300-A CCTTGCTCACCATGGTACCAAACCACTCATTTACAAACC 35S-F GTAAGGGATGACGCACAATC CsRPL1-OV-R ATTGAGAGAAGCGTTGATTTATGAG CsRPL2-OV-R TACTCAAATGTCGTAACCACTGCTT UBI-F CACCAAGCCCAAGAAGATC UBI-R TAAACCTAATCACCACCAGC Actin-F CCTTCGTCTTGATCTTGCGG Actin-R AGCGATGGCTGGAACAGAAC CsRPL1-qPCR-F CCGCTCACAATGGAAAACCC CsRPL1-qPCR-R AGGATACATTCAAGGGCTCG CsRPL2-qPCR-F TACGCCTCCTTTCGTTCCACT CsRPL2-qPCR-R TTGTCGTCGGAGATGTTAGGG CsRPL1-SP6 GATTTAGGTGACACTATAGAATGCTACCCGCTCACAATGGAAAAC CsRPL1-T7 TGTAATACGACTCACTATAGGGGTCCACCCATCACATACCCA CsRPL2-SP6 GATTTAGGTGACACTATAGAATGCTCTTCTTCTTTTTTTTCACCCG CsRPL2-T7 TGTAATACGACTCACTATAGGGCTTCCAAGGAGTCAAAAACGG