黄瓜幼苗下胚轴长度GWAS分析及候选基因挖掘

蔡和序,薄凯亮,周琪,苗晗,董邵云,顾兴芳,张圣平

GWAS Analysis of Hypocotyl Length and Candidate Gene Mining in Cucumber Seedlings

HeXu CAI,KaiLiang BO,Qi ZHOU,Han MIAO,ShaoYun DONG,XingFang GU,ShengPing ZHANG

表2 PCR反应程序

Table 2 PCR reaction procedure

热循环仪器 Thermal Cycler 时间和温度 Times and temperatures 初始步骤 Initial Steps 45个循环 Each of 45 cycles 解旋 Melt 退火 Anneal 延伸 Extend LightCycler480 Software Setup (罗氏 Roche) 95℃ 3 min 95℃ 15 s 57℃ 15 s 72℃ 30 s