黄瓜幼苗下胚轴长度GWAS分析及候选基因挖掘

蔡和序,薄凯亮,周琪,苗晗,董邵云,顾兴芳,张圣平

GWAS Analysis of Hypocotyl Length and Candidate Gene Mining in Cucumber Seedlings

HeXu CAI,KaiLiang BO,Qi ZHOU,Han MIAO,ShaoYun DONG,XingFang GU,ShengPing ZHANG

表1 荧光定量反应体系

Table 1 Fluorescence quantitative reaction system

反应组分 Reaction Component 浓度 Concentration 体积 Volume (µL) SybrGreen qPCR Master Mix 2× 10 引物F 10 µmol∙L-1 0.4 引物R 10 µmol∙L-1 0.4 ddH20 7.2 Template (cDNA) 2