食品动物源耐甲氧西林金黄色葡萄球菌MLSB类 抗生素耐药性调查
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李淑敏,方亮星,李亮,赵孟,陆肖,古伟琪,廖晓萍,孙坚,熊雁琼,刘雅红
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Investigation on the Antibiotic Resistance of Staphylococcus Methicillin-Resistant MLSB from Food Animals in Six Provinces of China
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LI ShuMin,FANG LiangXing,LI Liang,ZHAO Meng,LU Xiao,GU WeiQi,LIAO XiaoPing,SUN Jian,XIONG YanQiong,LIU YaHong
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表1 PCR引物序列
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Table 1 Primer sequences of the detection of MLSB resistant genes
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| 基因名称Genes’ name | 引物序列（5′→3′）Primer sequence | 片段长度Size (bp) | t退火 (℃) | 参考文献Reference | | mecA | F: AAAATCGATGGTAAAGGTTGGC
R: AGTTCTGCAGTACCGGATTTGC | 533 | 58 | [22] | | mecC | F:4GAAAAAAAGGCTTAGAACGCCTC
R:GAAGATCTTTTCCGTTTTCAGC | 137 | 58 | [23] | | ermA | F: GTTCAAGAACAATCAATACAGAG | 421 | 52 | [24] | | R: GGATCAGGAAAAGGACATTTTAC | | | ermB | F:CCGTTTACGAAATTGGAACAGGTAAAGGGC | 359 | 55 | [24] | | R: GAATCGAGAC TTGAGTGTGC | | | ermC | F: GCTAATATTG TTTAAATCGT CAATTCC | 572 | 52 | [25] | | R: GGATCAGGAA AAGGACATTT TAC | | | ereA | F: AACACCCTGAACCCAAGGGACG | 420 | 50 | [26] | | R: CTTCACATCCGGATTCGCTCGA | | | ereB | F: AGAAATGGAGGTTCATACTTACCA | 546 | 50 | [26] | | R: CATATAATCATCACCAATGGCA | |
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