转录因子CEBPα和p53在猪卵巢颗粒细胞中 对Kiss1基因表达的调控
辛晓萍1,王家迎1,张爱玲2,钟玉宜1,何颖婷1,陈赞谋1,张哲1,张豪1,李加琪1,袁晓龙1()
CEBPα and p53 Regulate Kiss1 Gene Expression in Porcine Ovary Granulosa Cells
XIN XiaoPing1,WANG JiaYing1,ZHANG AiLing2,ZHONG YuYi1,HE YingTing1,CHEN ZanMou1,ZHANG Zhe1,ZHANG Hao1,LI JiaQi1,YUAN XiaoLong1()

图2. 猪Kiss1基因上游区域区结合潜在转录因子生物信息学预测以及转录因子结合猪Kiss1基因上游区域的ChIP鉴定
M：DNA分子量标准（DL5 000 Marker）,从上到下依次为：5000,3000,2000,1500,1000,750,500,250和100 bp. IgG：阴性对照;Input and H3：阳性对照。A：猪Kiss1基因上游区域区结合潜在转录因子生物信息学预测结果;B：转录因子p53结合猪Kiss1基因上游区域的ChIP鉴定;C：转录因子CEBPα结合猪Kiss1基因上游区域的ChIP鉴定。其中,IgG：阴性对照;Input和H3：阳性对照

Fig. 2. Bioinformatics prediction for the potential binding site of transcription factors in the upstream region of porcine Kiss1, and ChIP results of transcription factors binding to the upstream region of porcine Kiss1 gene
M：DL5 000 DNA marker,from up to bottom：5000, 3000, 2000, 1500, 1000, 750, 500, 250, and 100 bp. IgG: Negative control; Input and H3: Positive control. A: The analysis of the binding sites for the transcription factor of the upstream region of porcine Kiss1 gene. B: The ChIP results for the binding of p53 with the upstream region of porcine Kiss1 gene. C: The ChIP results for the binding of CEBPα with the upstream region of porcine Kiss1 gene