葡萄霜霉病菌实时荧光定量PCR检测体系的建立和应用
李文学1,肖瑞刚2,吕苗苗1,丁宁1,石华荣2,顾沛雯1(
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Establishment and Application of Real-Time PCR for Quantitatively Detecting Plasmopara viticola in Vitis vinifera
LI WenXue1,XIAO RuiGang2,LÜ MiaoMiao1,DING Ning1,SHI HuaRong2,GU PeiWen1()
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图3. 引物F-Pv/R-Pv（A）和F-cox-Pv/R-Pv（B）常规PCR灵敏度检测
M：Marker;1—9：模板浓度依次为 50、5、1×100—1×10-6 ng·μL-1 The template concentration of 1-9 is 50, 5, 1×100-1×10-6 ng·μL-1;CK：清水对照Negative control group
Fig. 3. Sensitivity detection of P. viticola primers F-Pv/R-Pv (A) and F-cox-Pv/R-Pv (B) by using series genomic DNA dilutions with conventional PCR
