葡萄霜霉病菌实时荧光定量PCR检测体系的建立和应用

李文学¹,肖瑞刚²,吕苗苗¹,丁宁¹,石华荣²,顾沛雯¹(

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Establishment and Application of Real-Time PCR for Quantitatively Detecting Plasmopara viticola in Vitis vinifera

LI WenXue¹,XIAO RuiGang²,LÜ MiaoMiao¹,DING Ning¹,SHI HuaRong²,GU PeiWen¹(

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图1. 引物F-Pv/R-Pv（A）和F-cox-Pv/R-Pv（B）特异性检测
M：Marker;P1—P3：葡萄霜霉病菌P. viticola;1—13：12种病原菌和1种拮抗菌名称见表1 The names of 12 pathogenic fungi and 1 antagonistic fungus are listed in Table 1;CK：清水对照Negative control group

Fig. 1. Specificity detection of P. viticola primers F-Pv/R-Pv (A) and F-cox-Pv/ R-Pv (B) by using genomic DNA with conventional PCR