葡萄霜霉病菌实时荧光定量PCR检测体系的建立和应用

李文学,肖瑞刚,吕苗苗,丁宁,石华荣,顾沛雯

Establishment and Application of Real-Time PCR for Quantitatively Detecting Plasmopara viticola in Vitis vinifera

LI WenXue,XIAO RuiGang,LÜ MiaoMiao,DING Ning,SHI HuaRong,GU PeiWen

表2 潜伏侵染叶片中葡萄霜霉病菌的real-time PCR定量测定

Table 2 Quantitative determination of P. viticola in latent infected leaves by real-time PCR

接种后时间 Time after inoculation (h) Ct值 Ct value 检测结果 Detection result (copies/μL) 6 32.36±0.57 (5.68±2.76) ×104c 12 31.04±0.81 (2.12±1.17) ×105c 24 28.89±1.06 (1.26±0.72) ×106c 48 26.33±0.40 (5.95±1.52) ×106c 72 23.77±0.21 (2.62±0.37) ×107b 96 20.15±0.29 (1.30±0.20) ×108a