飞蝗LmGSTS2的酶学特性及其对马拉硫磷、 p,p’-DDT的代谢分析
马雯1,刘娇2,张学尧2,申国华3,秦雪梅1(
),张建琴1()
),张建琴1()
Enzymatic Characteristics and Metabolic Analysis to Malathion and p,p’-DDT of LmGSTS2 from Locusta migratoria
MA Wen1,LIU Jiao2,ZHANG XueYao2,SHEN GuoHua3,QIN XueMei1(),ZHANG JianQin1()
),ZHANG JianQin1()
图1. LmGSTS2的表达及纯化
Fig. 1. Expression and purification of LmGSTS2
M：10—180 kD蛋白分子量标准 10-180 kD protein molecular weight standards。A：LmGSTS2的表达 Expression of LmGSTS2。1：BL21(DE3);2：pET28a/BL21(DE3);3：未诱导pET28a/BL21(DE3)-LmGSTS2 pET28a/BL21(DE3)-LmGSTS2 before induction;4：诱导后的pET28a/BL21(DE3)-LmGSTS2 pET28a/BL21(DE3)-LmGSTS2 after induction。B：LmGSTS2的纯化 Purification of LmGSTS2。 1：未纯化的LmGSTS2总蛋白Total protein of LmGSTS2 before purification;2：过柱穿透液 Flow through fraction;3—12：不同浓度咪唑缓冲液洗脱组分Elution components with different concentrations of imidazole buffer
