酮类物质合成酶OsPKS1和OsPKS2对水稻花粉外壁形成的作用
周雨露1,林泓1,张大兵1,2,王灿华1(
),余婧1()
),余婧1()
The Function of the Polyketide Synthase OsPKS1 and OsPKS2 in Regulating Pollen Wall Formation in Rice
ZHOU YuLu1,LIN Hong1,ZHANG DaBing1,2,WANG CanHua1(),YU Jing1()
),YU Jing1()
图1. OsPKS1 gRNA靶位点及突变位点分析
A：2个靶点在OsPKS1中的位置及2个靶点gRNA表达盒与pRGEB32CRISPR/Cas9组装成的表达载体;B：ospks1-3和ospks1-4 ospks2突变体的突变位点测序结果;C：野生型与突变体表达蛋白分析,色框显示的是预测出的保守域,箭头表示突变位置。D：其他等位的基因型信息
Fig. 1. Target sites of the gRNA in the OsPKS1 gene and mutation site analysis
A: Position of two targets in OsPKS1 gene locus, cloning of two gRNA cassettes into the pRGEB32CRISPR/Cas9 vector; B: Mutation site sequencing results of ospks1-3 and ospks1-4 ospks2; C: The protein prediction analysis of the wild type and the mutants, green boxes indicate putative conserved domain, the arrows indicate the mutation position. D: Mutation information in other alleles
