F17大肠杆菌在湖羊羔羊个体脾脏中LncRNA表达谱变化

黄赛男,金澄艳,鲍建军,王悦,陈炜昊,吴天弋,王利宏,吕晓阳,高雯,王步忠,朱国强,戴国俊,孙伟

Changes of LncRNA Expression Profile in Spleen of Diarrhea and Non-diarrhea Individuals in F17 of Hu Sheep Lamb

HUANG SaiNan,JIN ChengYan,BAO JianJun,WANG Yue,CHEN WeiHao,WU TianYi,WANG LiHong,LÜ XiaoYang,GAO Wen,WANG BuZhong,ZHU GuoQiang,DAI GuoJun,SUN Wei

表3 GAPDH,DE lncRNA和mRNA的引物

Table 3 The primer of GAPDH, DE lncRNAs and mRNAs

	基因符号Gene symbol	引物序列Primer sequence	产物长度Product length (bp)
10个DE mRNA的引物Primers of ten DE mRNA	XM_015103189.1	F:AGCACTTCCTCCTGTCCG	132
	XM_015103189.1	R:CAGCACAGAAGGCAAAGTC	132
	XM_012106626.2	F:CCGAGTTTGCAGGTACCCAAC	121
	XM_012106626.2	R:TTTTGGCGCATGTATACCTG	121
	XM_012095567.2	F:TGAGACTCTACTTCGCTGC	102
	XM_012095567.2	R:TTGCCCATCCTTAATAGCTG	102
	XM_012181407.2	F:ACGACGGTGGTTAAATACTC	125
	XM_012181407.2	R:AGTTGCCCATAGTCACTGGTC	125
	XM_012181919.2	F:TCAACCATATGCTGACGGAC	119
	XM_012181919.2	R:ATGCCGCCTATCAAGGTC	119
	XM_012152506.2	F:CCACCTGCGGTTCAAGTTAC	157
	XM_012152506.2	R:TGCCTGAATCACCTTGTC	157
10个DE lncRNA的引物 Primers of ten DE lncRNA	TCONS_00053949	F:CCTGGCTATATCCTTACATCAC	100
	TCONS_00053949	R:AAGTTCAAACTCCGCTGCAC	100
	TCONS_00059692	F:AATTTCTTCTCGTTCCAAGGC	131
	TCONS_00059692	R:CCAACAGGGAGCCAACTTC	131
	TCONS_00089751	F:AGAAGGCTTTGACCGAAC	153
	TCONS_00089751	R:TCAATGCCCTCCACGAGAC	153
	TCONS_00099925	F:AGTGCCACATGTACCTAGCAG	146
	TCONS_00099925	R:ACGACAGGCATTTTAACCCATG	146
	TCONS_00107469	F:TAGTACAGCCCATATTTATCG	115
	TCONS_00107469	R:ATTTTCTTTCCACAGGGACG	115
	TCONS_00099739	F:CCGACGCTGTCATGATGC	159
	TCONS_00099739	R:TCCGTCTCCAGAACCAAGGC	159
GAPDH的引物 Primers of GAPDH	GAPDH-F	F:GTTCCACGGCACAGTCAAGG	127
GAPDH的引物 Primers of GAPDH	GAPDH-F	R:ACTCAGCACCAGCATCACCC	127