Krüppel-Like Factor 3（KLF3）基因对牛脂肪沉积的作用
|
|
郭红芳,宁越,成功,昝林森
|
The Effect of Krüppel-Like Factor 3 (KLF3) Gene on Bovine Fat Deposition
|
|
GUO HongFang,NING Yue,CHENG Gong,ZAN LinSen
|
|
表1 试验中所用引物
|
Table 1 Primer used in the study
|
|
| 引物Primer | 引物序列（5′-3′）Sequence（5′-3′） | 片段大小Fragment size (bp) | Tm（℃） | | KLF3-Si2-F | GCAAAGGAAGCGGAGAAUATT | — | — | | KLF3-Si2-R | UAUUCUCCGCUUCCUUUGCTT | — | — | | KLF3-Si3-F | GGAAACACACUGGAAUCAATT | — | — | | KLF3-Si3-R | UUGAUUCCAGUGUGUUUCCTT | — | — | | PPARγ-F | GAGATCACAGAGTACGCCAAG | 216 | 60 | | PPARγ-R | GGGCTCCATAAAGTCACCAA | | CEBPα-F | ATCTGCGAACACGAGACG | 73 | 60 | | CEBPα-R | CCAGGAACTCGTCGTTGAA | | ACCα-F | CTCCAACCTCAACCACTACGG | 171 | 60 | | ACCα-R | GGGGAATCACAGAAGCAGCC | | FAS-F | TAAGGTTCAAATTGCTGCGT | 138 | 60 | | FAS-R | TCCAGAGCGAAGGAGAGATT | | FABP4-F | TGAGATTTCCTTCAAATTGGG | 101 | 60 | | FABP4-R | CTTGTACCAGAGCACCTTCATC | | GAPDH-F | CCAACGTGTCTGTTGTGGAT | 80 | 60 | | GAPDH-R | CTGCTTCACCACCTTCTTGA | | KLF3-F | CAGTCCCTGTCAAGCAAGAG | 111 | 60 | | KLF3-R | ACAATGGCGTGGAGTAGATG |
|
|
|