短期生物炭添加对不同类型土壤细菌和氨氧化微生物的影响

张梦阳,夏浩,吕波,丛铭,宋文群,姜存仓

Short-Term Effect of Biochar Amendments on Total Bacteria and Ammonia Oxidizers Communities in Different Type Soils

ZHANG MengYang,XIA Hao,LÜ Bo,CONG Ming,SONG WenQun,JIANG CunCang

表5 土壤氨合成相关酶和氨氧化作用相关酶基因表达量

Table 5 Gene expression of soil ammonia synthesis related enzymes and ammonia oxidation related enzymes

酶 Enzyme EC（编号）EC (number) YB FA B CK F C FC CK F C FC CK F C FC 甲酰胺酶 Formamidase 1.7.2.1 1073a 1384a 1014a 1083a 1077a 1069a 892a 933a 361b 328b 329b 477a 腈水解酶 Nitrilase 3.5.1.49 4513ab 5000a 4044b 4582ab 5402a 6062a 5352a 5025a 5849a 5448a 5232a 6008a 氰酸裂合酶 Cyanate lyase 3.5.5.1 7036ab 7303ab 5903b 7985a 9535a 8958a 9566a 9398a 9240ab 8538bc 8168c 9710a 氨基甲酸酯激酶 Carbamate kinase 4.2.1.104 4006ab 3632b 3521b 4448a 4539a 4761a 4592a 4837a 4657a 4547a 4384a 4533a 谷氨酸脱氢酶 Glutamate dehydrogenase 2.7.2.2 4984ab 5332a 4574ab 4200b 5148a 5068a 3263b 3092b 9561a 9745a 10561a 10095a 谷氨酸脱氢酶(NAD(P)+) Glutamate dehydrogenase ((NAD(P)+) 1.4.1.2 9631ab 10188a 8444b 9389ab 10659a 11757a 8684b 8277b 17594a 16898a 16405a 16705a 谷氨酸脱氢酶(NADP+) Glutamate dehydrogenase (NADP+) 1.4.1.3 43350a 42063a 41901a 35676a 43520a 39070b 36778b 37143b 29553a 32706a 35500a 32690a 亚硝酸还原酶 (不分解) Nitrite reductase (No-forming) 1.4.1.4 13424a 11436a 10945a 12922a 12085bc 14166a 13367ab 10763c 13604a 13240a 14048a 13256a