褐飞虱GSK-3调控糖原与海藻糖代谢的潜在功能

丁艳娟,刘永康,罗雨嘉,邓颖梅,徐红星,唐斌,徐彩娣

Potential Functions of Nilaparvata lugens GSK-3 in Regulating Glycogen and Trehalose Metabolism

DING YanJuan,LIU YongKang,LUO YuJia,DENG YingMei,XU HongXing,TANG Bin,XU CaiDi

表1 dsRNA合成及荧光定量PCR引物

Table 1 dsRNA synthesis and qRT-PCR primers

引物名称Primer name 引物序列Primer sequence (5′-3′) 引物名称Primer name 引物序列Primer sequence (5′-3′) GSK-3 F: GGAAAGTTGAATCAAAGTGCTCG R: AGGCTTTTGCCAGGGATG qNlIlp1 F: AACGATGCTGACTTGCAGATT R: CGTACACGCGGAATAAATCA dsGSK-3 F: T7-CTGCGACAGCGGCGAAATG R: T7-CGGTGACAGATGCCCAGCGAGT qNlIlp2 F: TTCTCAGCCGCTCTAGCAAT R: CAGACGAAGGATCAGGGAAG dsGFP F: T7-AAGGGCGAGGAGCTGTTCACCG R: T7-CAGCAGGACCATGTGATCGCGC qNlIlp3 F: ATACTGCGGCCAATAGCAAG R: TCTCAATCCCCAAAATCAGC qNlTRE1-1 F: GCCATTGTGGACAGGGTG R: CGGTATGAACGAATAGAGCC qNlIlp4 F: TCCCGGACAGTTCTCACTTT R: TTGTATTCTCCGGAGGCAAG qNlTRE1-2 F: GATCGCACGGATGTTTA R: AATGGCGTTCAAGTCAA qNlTPS1 F: AAGACTGAGGCGAATGGT R: AAGGTGGAAATGGAATGTG qNlTRE2 F: TCACGGTTGTCCAAGTCT R: TGTTTCGTTTCGGCTGT qNlTPS2 F: AGAGTGGACCGCAACAACA R: TCAACGCCGAGAATGACTT qNlInR1 F: GAGTGCAACCCGGAGTATGT R: TCTTGACGGCACACTTCTTG qNlGP F: GCTGCCTATGGCTATGGTATTC R: TCTGAGTGTTGACCCACTTCTTG qNlInR2 F: CTCTTGCCGAACAGCCTTAC R: GGGTCGTTTAGTGGGTCTGA qNlGS F: GCTCCAAAGCCTATGTTTCTACTG R: TGGTAACCCCTGTCCCTCA T7 GGATCCTAATACGACTCACTATAGG qNl18S F: CGCTACTACCGATTGAA R: GGAAACCTTGTTACGACTT