不同糖源对葡萄试管苗蛋白激酶相关基因表达的影响
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梁国平,李文芳,陈佰鸿,左存武,马丽娟,何红红,万鹏,安泽山,毛娟
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Effects of Different Sugar Sources on Protein Kinase Gene Expression in Grape Plantlets
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LIANG GuoPing,LI WenFang,CHEN BaiHong,ZUO CunWu,MA LiJuan,HE HongHong,WAN Peng,AN ZeShan,MAO Juan
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表1 qRT-PCR引物
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Table 1 qRT-PCR primer
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| 基因号 Gene ID | 引物序列（5′-3′） Primer sequence (5′-3′) | | GSVIVG01014744001 | F-TCAAGGACATCACCACCACA | R-TAGGCCCTTTACGACACACA | | GSVIVG01015297001 | F-ATAGAAGACGCGGTTGGACA | R-TACCCAAGATCACTGCAGCA | | GSVIVG01014081001 | F-AAGACAAGCAGCCATCTCCT | R-TGTGCCTGCAACAGCTTTAG | | GSVIVG01019739001 | F-AGGATTGGTTGAAGGCTCCA | R-AAGGCGTGACTCAGATGGAT | | GSVIVG01026984001 | F-TTGAAGGGGCACTGTCTCAT | R-ACTGGTGGGTCTGGATTGAA | | GSVIVG01038192001 | F-AGCCTCCAATCATGCCCATA | R-ATGATCCATGTGCCGCAAAA | | GSVIVG01038760001 | F-ACGTTAGTGGAGGGAATGCT | R-TGTCCCAATGTTCTCCCCAT | | GSVIVG01001347001 | F-TGGCCCTGAAAGTCCGTTAT | R-TTCAACCAAGGCCGTCAATG | | GSVIVG01020128001 | F-GGAAGCAGCAATAACGTGGT | R-TCTGCCAAGAGACAACCCAA | | GSVIVG01025420001 | F-GGGTTGGGTGCTGTTTTCAA | R-TGCCTCCTTATGCCGAAACT | | GSVIVG01038559001 | F-GGGGTCCAAATTCTTTCGCA | R-TTCCTCGCCATCATCATCCA | | GSVIVG01000919001 | F-TTCGGGCAAGTTTTGGAAGG | R-AAAAGGGAGAAGGAGGTGGA | | GSVIVG01011043001 | F-GGGTTGGACGGCTGAATTTT | R-GGAGGAACGAAACAATGGCA | | GSVIVG01020071001 | F-ATGAGGTTGGCTGCTACTGA | R-AAGGCAAGAACACTGTCCCT | | GSVIVG01000561001 | F-TCTTGGGCTTGGATGGAGTT | R-AGCATGTCGGTCCACTCTTT | | GSVIVG01007646001 | F-AGGCATGGAGAGAAGAAGCA | R-TCTGGGAGGTTTTCAGCACT | | GSVIVG01009192001 | F-AGCGAGGGTGTAAGGTTTGT | R-TCAGCAGTCACATCCTTGGT | | GSVIVG01009685001 | F-ACGGAACTCAGCGTATCAGT | R-TCGTCATCATCGGGTTTCCA | | GSVIVG01013564001 | F-GTTCCGGCCAAATATGAGCA | R-TTGACCGGAAGAAATTCGCC | | GSVIVG01014110001 | F-TGTAGCTGCACCAACAACAC | R-TGGAGGGATTGAACCGTTGA | | GSVIVG01014128001 | F-AGGCTGCCACAAACAACTTT | R-TGCGGATGTTGTAGAGCAGA | | GSVIVG01023376001 | F-TGGACTGTGAGGCTAAACGT | R-TTTAAAAGCCTAGCCGTGCC | | GSVIVG01023676001 | F-TCATCATCCTCATCGCCATCA | R-AACTTGTCGCATGGTTGGTC | | GSVIVG01026000001 | F-AGCGTTTGGGGACTTGAGTA | R-TCCCAGAGCAAGCAAGTACA | | GSVIVG01026487001 | F-TGACGGGAAAGCAGGAAGAA | R-AAACATGCCCAAAAGTCCCC | | GSVIVG01029835001 | F-TGGCTTACGGAGGTGAACTT | R-AGCACCCAGAGCAATCATCA | | GSVIVG01032611001 | F-TTCCCCACCCATCATTCACA | R-TACGGTCATGGTGTAGGCAA | | GAPDH | F-TTCTCGTTGAGGGCTATTCCA | R-CCACAGACTTCATCGGTGACA |
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