水稻广谱抗稻瘟病基因PigmR功能标记的开发及应用

王芳权,陈智慧,许扬,王军,李文奇,范方军,陈丽琴,陶亚军,仲维功,杨杰

Development and Application of the Functional Marker for the Broad-Spectrum Blast Resistance Gene PigmR in Rice

WANG FangQuan,CHEN ZhiHui,XU Yang,WANG Jun,LI WenQi,FAN FangJun,CHEN LiQin,TAO YaJun,ZHONG WeiGong,YANG Jie

表1 分子标记引物

Table 1 The primers of molecular marker

标记名称 Marker name 引物名称 Primer name 引物序列 Primer sequence (5′-3′) 理论产物长度 Expected size (bp) GMR-1 GMR-1F TTCCTTTCTCTTTATCATAATT 180 GMR-1R AGATTCCAACCTGCACTTGCCT GMS-1 GMS-1F GTCCTTGATCTTTATCAGAAAG 180 GMS-1R TGGTTCCAACCTGCAGAATCTG GMR-2 GMR-2F TTCCTTTCTCTTTATCATAcTT 180 GMR-2R AGATTCCAACCTGCACTTGCCT GMS-2 GMS-2F GTCCTTGATCTTTATCAGAcAG 180 GMS-2R TGGTTCCAACCTGCAGAATCTG GMR-3 GMR-3F AGTTCTACTTACGGAGGAGC 98 GMR-3R AGAATTATGATAAAGAGAAAGGAA GMS-3 GMS-3F AGATCAACTTATTGAGCAGC 98 GMS-3R AGCTTTCTGATAAAGATCAAGGAC GMR-4 GMR-4F AGTTCTACTTACGGAGGcGC 98 GMR-4R AGAATTATGATAAAGAGAAAGcAA GMS-4 GMS-4F AGATCAACTTATTGAGCcGC 98 GMS-4R AGCTTTCTGATAAAGATCAAGcAC Actin1-1 Actin1-1F ACAAAGTTTTCAACCGGCCTA 146 Actin1-1R CTGGTACCCTCATCAGGCATC Actin1-2 Actin1-2F AGTCTGGCCCATCCATTGTG 149 Actin1-2R CGGTTGAAAACTTTGTCCACGCTA