感染大肠杆菌F17湖羊羔羊脾脏中差异circRNA分析

邹双霞,金澄艳,鲍建军,王悦,陈炜昊,吴天弋,王利宏,吕晓阳,高雯,王步忠,朱国强,戴国俊,师东方,孙伟

Differential circRNA Analysis in the Spleen of Hu-sheep Lambs Infected with F17 Escherichia coli

ZOU ShuangXia,JIN ChengYan,BAO JianJun,WANG Yue,CHEN WeiHao,WU TianYi,WANG LiHong,LÜ XiaoYang,GAO Wen,WANG BuZhong,ZHU GuoQiang,DAI GuoJun,SHI DongFang,SUN Wei

表1 GAPDH,DE circRNA和mRNA的引物

Table 1 The primer of GAPDH, DE circRNAs and mRNAs

基因符号Gene symbol 引物序列Primer sequence 产物长度Length of product (bp) 差异表达的环状RNA的引物 Primers of DE circRNA circRNA_2125 F:ATTGAATCACTTCTCTGTTGC 129 R:TAGGTGCTCAAAATAGGAC circRNA_3832 F:AGCCTCTCATCTGTACAC 134 R:CAGTAACTGCCTAGAGCA circRNA_7711 F:ACAAAGATTCCATTGACAG 101 R:ACCAAGAGGCTAGCAAGAC circRNA_6710 F:CAGATTACAGCTATGGCGA 124 R:CCCTCATGATCTCATAGG circRNA_6914 F:TTGGCTGTTACTATCATGAG 124 R:CTGAACTCTTAACTTGCA circRNA_4030 F:TGATGCAGATATTAAACCTC 133 R:CCAATCTCGGATAACTTCAC 差异表达的信使RNA的引物 Primers of DE mRNA NEB F:ATTACAGCTATCCACCCGAC 149 R:TGCCTTTTCCATTTCTAAG UBE3B F:TAAGATTGCCAGGAAACTGC 133 R:AGCCAGGGACACGTACCAC ADGRF2 F:GGCGTTTACCTCTTTCTCG 103 R:CAAGCTGCAAATAGAAAC LAMA1 F:AAATGATCGAAAAGGCTAC 127 R:AACCGCCTTTTCCGTAGGAC LTF F:GAAAAGCGTATCCCAACCTG 103 R:TTGAAGGCACCAGAATAAC MGAT5 F:CATCATCCACACCTACACG 111 R:AACTGCAAGTCTCGTCCGC TLN2 F:ACGACGGTGGTTAAATAC 125 R:AGTTGCCCATAGTCACTGGTC ARHGAP30 F:TCTTCAACCTGGGTCGCTC 159 R:GCAGCCCCTCTGGTTCATC SLC25A29 F:GCGTCCTGGCTCTCCACCT 125 R:CCCTGCCTCCCCGCGCTC GAPDH引物 Primers of GAPDH GAPDH-F F:GTTCCACGGCACAGTCAAGG 127 R:ACTCAGCACCAGCATCACCC