无刺蜂蜂胶乙醇提取物的体外抗氧化及抗炎活性

王蓓,常化松,苏松坤,孙丽萍,王凯

Antioxidative and Anti-inflammatory Activities of Ethanol Extract of Geopropolis from Stingless Bees

WANG Bei,CHANG HuaSong,SU SongKun,SUN LiPing,WANG Kai

表1 实时荧光定量PCR相关引物序列

Table 1 The primer sequence of related genes in RT-qPCR

基因Gene 上游引物序列Upstream primer sequence 下游引物序列Downstream primer sequence INOS 5′-TTTCCAGAAGCAGAATGTGACC-3′ 5′-AACACCACTTTCACCAAGACTC-3′ HO-1 5′-ACATTGAGCTGTTTGAGGAG-3′ 5′-TACATGGCATAAATTCCCACTG-3′ IL-1β 5′-CCAACAAGTGATATTCTCCATGAG-3′ 5′-ACTCTGCAGACTCAAACTCCA-3′ IL-10 5′-CTATGCTGCCTGCTCTTACTG-3′ 5′-CAACCCAAGTAACCCTTAAAGTC-3′ GAPDH 5′-GAGAAACCTGCCAAGTATGATGAC-3′ 5′-TAGCCGTATTCATTGTCATACCAG-3′