青枯菌铜抗性基因copA 的功能
王晓宁,梁欢,王帅,方文生,许景升,冯洁,徐进(),曹坳程()
Function of Copper-Resistant Gene copA of Ralstonia solanacearum
WANG XiaoNing,LIANG Huan,WANG Shuai,FANG WenSheng,XU JingSheng,FENG Jie,XU Jin(),CAO AoCheng()

图 8. Biolog代谢芯片测定
紫色孔定义为阳性,浅于紫色的孔定义为边界值。该图分别表示野生型菌株、基因缺失菌株ΔPo82copA和互补菌株pBBR-ΔPo82copA在培养96 h后的代谢结果。α -D-葡萄糖(微孔板C1)在3种菌株的OD590分别为0.632、0.521、0.372;D-海藻糖(微孔板A4)在3种菌株的OD590分别为0.620、0.480、0.383;L-丙氨酸(微孔板E3)在3种菌株的OD590分别为0.786、0.370、0.436;葡萄糖醛酰胺(微孔板F6)在3种菌株的OD590分别为0.566、0.388、0.380

Fig. 8. Biolog metabolic microarray detection
Purple pore is defined as positive and pores shallower than purple are defined as boundary values. The picture indicates the metabolic results of Po82, ΔPo82copA and pBBR-ΔPo82copA strains in 96 h, respectively. The OD590 of α -D-glucose (micropore plate C1) in the three strains is 0.632, 0.521 and 0.372, respectively. The OD590 of D-trehalose (micropore plate A4) in the three strains is 0.620, 0.480 and 0.383, respectively. The OD590 of L-alanine (micropore plate E3) in the three strains is 0.786, 0.370 and 0.436, respectively. The OD590 of glucuronamide (micropore plate F6) in the three strains is 0.566, 0.388 and 0.380, respectively