青枯菌铜抗性基因copA 的功能
王晓宁,梁欢,王帅,方文生,许景升,冯洁,徐进(
),曹坳程()
),曹坳程()
Function of Copper-Resistant Gene copA of Ralstonia solanacearum
WANG XiaoNing,LIANG Huan,WANG Shuai,FANG WenSheng,XU JingSheng,FENG Jie,XU Jin(),CAO AoCheng()
),CAO AoCheng()
图3. copA 基因缺失及互补菌株PCR检测
A：缺失载体酶切验证图The enzyme cutting verification diagram of deletion plasmid;B：互补载体验证图（copA Testf/r）The verification diagram of complementary carrier (copA Testf/r);C：敲除及互补菌株引物检测（759f/760r;1—4：Po82野生型菌株、copA 缺失菌株、copA互补菌株、空白对照）The detection of deletion and complementary strains (759f/760r; 1-4: Po82 wild-type strain, copA gene deletion strain, copA complementary strain and the control);D：敲除及互补菌株引物检测（copAf/r;1—4：Po82野生型菌株、copA 互补菌株、copA 缺失菌株、空白对照）The detection of deletion and complementary strains (copAf/r; 1-4: Po82 wild-type strain, copA complementary strain, copA gene deletion strain and the control)
Fig. 3. PCR detection of copA gene deletion and complementary strains
