青枯菌铜抗性基因copA 的功能
王晓宁,梁欢,王帅,方文生,许景升,冯洁,徐进,曹坳程

Function of Copper-Resistant Gene copA of Ralstonia solanacearum
WANG XiaoNing,LIANG Huan,WANG Shuai,FANG WenSheng,XU JingSheng,FENG Jie,XU Jin,CAO AoCheng
表2 本试验所用引物序列
Table 2 Primer sequences used in this study
引物名称 Primer name 引物序列 Primer sequence (5′-3′) 用途Purpose
759f GTCGCCGTCAACTCACTTTCC 青枯菌特异性验证
Specificity verification of R. solanacearum
760r GTCGCCGTCAGCAATGCGGAATCG
copA Upf GGTCTTAAUCCCGTGAGGTTGGGAGGTGA 扩增copA 上游片段
Amplification of upstream of copA
copA Upr GGCATTAAUTGCTCGACTTTCACAGCGGA
copA Downf GGACTTAAUGATCGAGTTTTCCTTACGTAA 扩增copA 下游片段
Amplification of downstream of copA
copA Downr GGGTTTAAUTCACGCAGCGCCGTGTAGTCC
copAf ATATGGCGGCCTCATCATCG 基因缺失菌株验证
Verification of gene deletion strain
copAr CGAGAAAAGCCCTGTCCAGT
pBRRf CATTAGGCACCCCAGGCTTTAC 质粒线性化
Plasmid linearization
pBRRr CCTCTTCGCTATTACGCCAGC
copA Hf CCTCGAGGTCGACGGTATCGATATGCGCAGCAATCGTGCATCCCGCC copA 扩增
Amplification of copA
copA Hr GAACTAGTGGATCCCCCGGGCTTCAGGCCACCACCACTTCGCGGAAC
copA Testf GCCGTTTGTGATGGCTTCC 基因互补菌株验证
Verification of gene complementary strain
copA Testr CTTATTCAGGCGTAGCACCAGG
gyrBf GACCTTCCAGGGGTTGATCG RT-qPCR
gyrBr TCTCCCCCAGCCCCTTATAC
copAf ATATGGCGGCCTCATCATCG
copAr CGAGAAAAGCCCTGTCCAGT
hrpBf GAAGTGGCCGCCCATATC
hrpBr GCTTGCGGTAGCCCTTGA
hrpGf GGACACATTCCACGTTCTGCA
hrpGr CCATGAAATTCGCCGTATTGA
popAf TTCAGGAGCTTCACCAGGTCT
popAr CAACACCAATGGCAACTCCAA