青枯菌铜抗性基因copA 的功能

王晓宁,梁欢,王帅,方文生,许景升,冯洁,徐进,曹坳程

Function of Copper-Resistant Gene copA of Ralstonia solanacearum

WANG XiaoNing,LIANG Huan,WANG Shuai,FANG WenSheng,XU JingSheng,FENG Jie,XU Jin,CAO AoCheng

表2 本试验所用引物序列

Table 2 Primer sequences used in this study

引物名称 Primer name 引物序列 Primer sequence (5′-3′) 用途Purpose 759f GTCGCCGTCAACTCACTTTCC 青枯菌特异性验证 Specificity verification of R. solanacearum 760r GTCGCCGTCAGCAATGCGGAATCG copA Upf GGTCTTAAUCCCGTGAGGTTGGGAGGTGA 扩增copA 上游片段 Amplification of upstream of copA copA Upr GGCATTAAUTGCTCGACTTTCACAGCGGA copA Downf GGACTTAAUGATCGAGTTTTCCTTACGTAA 扩增copA 下游片段 Amplification of downstream of copA copA Downr GGGTTTAAUTCACGCAGCGCCGTGTAGTCC copAf ATATGGCGGCCTCATCATCG 基因缺失菌株验证 Verification of gene deletion strain copAr CGAGAAAAGCCCTGTCCAGT pBRRf CATTAGGCACCCCAGGCTTTAC 质粒线性化 Plasmid linearization pBRRr CCTCTTCGCTATTACGCCAGC copA Hf CCTCGAGGTCGACGGTATCGATATGCGCAGCAATCGTGCATCCCGCC copA 扩增 Amplification of copA copA Hr GAACTAGTGGATCCCCCGGGCTTCAGGCCACCACCACTTCGCGGAAC copA Testf GCCGTTTGTGATGGCTTCC 基因互补菌株验证 Verification of gene complementary strain copA Testr CTTATTCAGGCGTAGCACCAGG gyrBf GACCTTCCAGGGGTTGATCG RT-qPCR gyrBr TCTCCCCCAGCCCCTTATAC copAf ATATGGCGGCCTCATCATCG copAr CGAGAAAAGCCCTGTCCAGT hrpBf GAAGTGGCCGCCCATATC hrpBr GCTTGCGGTAGCCCTTGA hrpGf GGACACATTCCACGTTCTGCA hrpGr CCATGAAATTCGCCGTATTGA popAf TTCAGGAGCTTCACCAGGTCT popAr CAACACCAATGGCAACTCCAA