青枯菌铜抗性基因<i>copA</i> 的功能

青枯菌铜抗性基因copA 的功能

王晓宁,梁欢,王帅,方文生,许景升,冯洁,徐进,曹坳程

Function of Copper-Resistant Gene copA of Ralstonia solanacearum

WANG XiaoNing,LIANG Huan,WANG Shuai,FANG WenSheng,XU JingSheng,FENG Jie,XU Jin,CAO AoCheng

表1 本试验所用菌株及质粒

Table 1 Strains and plasmids used in this study

菌株和质粒 Strains and plasmids		特征 Characteristics	来源 Source
菌株 Strain	R. solanacearum Po82	Wild-type potato strain; Phylotype Ⅱ, sequevar. 4 (race 3 biovar. 2)	本实验室The laboratory
	E. coli DH5α	mcrA φ80 lacZΔM15, recA1, endA1	本研究This study
	ΔPo82copA	copA deletion strain, Gm^r	本研究This study
	PBBR-ΔPo82copA	copA complentmentary strain, Gm^r, Amp^r	本研究This study
质粒 Plasmid	pKMS1-gm (+U)	Cloning vector, Gm^r, Kan^r	本实验室The laboratory
	pBBR1MCS-4	Amp^r, lacZ alpha	本实验室The laboratory
	pKMS1-copA-gm	Gm^r, for copA gene deletion	本研究This study
	pBBR-copA	Amp^r, for copA gene complementation	本研究This study