黄淮海地区夏玉米弯孢叶斑病菌遗传多样性分析
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常佳迎,刘树森,马红霞,石洁,郭宁,张海剑
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Genetic Diversity Analysis of Curvularia lunata in Summer Maize in Huang-Huai-Hai Region
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CHANG JiaYing,LIU ShuSen,MA HongXia,SHI Jie,GUO Ning,ZHANG HaiJian
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表2 ISSR引物扩增
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Table 2 The amplification result with used primers
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引物
Primer | 序列
Sequence (5′-3′) | 退火温度
Tm (℃) | 扩增条带数
Number of amplified bands | 多态性条带
Number of polymorphic bands | 多态性比率
Polymorphism ratio (%) | | 808 | (AG)8C | 49 | 10 | 10 | 100 | | 810 | (GA)8T | 45 | 7 | 7 | 100 | | 823 | (TC)8C | 50 | 6 | 6 | 100 | | 827 | (AC)8G | 52 | 7 | 7 | 100 | | 835 | (AG)8YC | 53 | 10 | 10 | 100 | | 841 | (GA)8YC | 50 | 11 | 11 | 100 | | 842 | (GA)8YG | 50 | 10 | 10 | 100 | | 847 | (CA)8RC | 54 | 6 | 6 | 100 | | 855 | (AC)8YT | 46 | 7 | 7 | 100 | | 873 | (GACA)4 | 49 | 7 | 7 | 100 | | 880 | (GGAGA)3 | 46 | 7 | 7 | 100 | | 888 | BDB(CA)7 | 48 | 9 | 9 | 100 | | 891 | HVH(TG)7 | 47 | 8 | 8 | 100 | | 总计Total | | | 105 | 105 | 100 |
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