中华蜜蜂Apidaecin的重组表达及其抗菌活性

陈文凤,王红芳,刘振国,张卫星,郗学鹏,胥保华

Recombinant Expression and Antimicrobial Activity of Apidaecin in Apis cerana cerana

CHEN WenFeng,WANG HongFang,LIU ZhenGuo,ZHANG WeiXing,CHI XuePeng,XU BaoHua

表2 重组抗菌肽Apidaecin对小鼠感染大肠杆菌K88保护作用试验分组

Table 2 Groups for test the protective effects of recombinant Apidaecin on mice infected with E. coli K88

分组 Group 步骤1 Step 1 （第1—6天腹腔注射 Intraperitoneal injection at 1st-6th days） 步骤2 Step 2 （第6天腹腔注射后1 h再注射 Repeated intraperitoneal injection was performed 1 h after the first injection at 6th day） A：正常对照组 Normal control group 0.5 mL生理盐水0.5 mL Saline 0.5 mL生理盐水0.5 mL Saline B：pHT43空载体 pHT43 Empty carrier 0.5 mL pHT43 0.5 mL生理盐水 0.5 mL Saline C：染菌对照组Infection control group 0.5 mL生理盐水0.5 mL Saline 0.5 mL菌悬液0.5 mL Bacteria suspension D：pHT43空载体染菌组 pHT43 Empty carrier infection group 0.5 mL pHT43 0.5 mL菌悬液0.5 mL Bacteria suspension E：20 mg·L-1重组Apidaecin染菌组 20 mg·L-1 Recombinant Apidaecin infection group 0.5 mL重组Apidaecin 0.5 mL Recombinant Apidaecin 0.5mL菌悬液0.5 mL Bacteria suspension F：150 mg·L-1头孢噻肟染菌组 150 mg·L-1 Cefotaxime infection group 0.5 mL头孢噻肟溶液 0.5 mL Cefotaxime solution 0.5 mL菌悬液0.5 mL Bacteria suspension