中华蜜蜂Apidaecin的重组表达及其抗菌活性

陈文凤,王红芳,刘振国,张卫星,郗学鹏,胥保华

Recombinant Expression and Antimicrobial Activity of Apidaecin in Apis cerana cerana

CHEN WenFeng,WANG HongFang,LIU ZhenGuo,ZHANG WeiXing,CHI XuePeng,XU BaoHua

表1 基因引物序列

Table 1 Primer sequences of gene

基因名称 Gene name 引物序列 Primer sequence (5′-3′) 引物用途 Description 登录号 GenBank accession number API-F TGTGGGTTGAATAACTATTGATAA cDNA sequence primer, forward NM_001011613.1 API-R GTTATTTCACGTGCTTCATATTC cDNA sequence primer, reverse GAPDH-F GGTTGTCTCCTGCGACTTCA Standard control primer, forward NM_001289726.1 GAPDH-R TGGTCCAGGGTTTCTTACTCC Standard control primer, reverse IL10-F ACTGCTATGCTGCCTGCTCT Real-time PCR primer, forward NM_010548.2 IL10-R GACTGGGAAGTGGGTGCAGT Real-time PCR primer, reverse TNF-α-F CCAGCCGATGGGTTGTACCT Real-time PCR primer, forward NM_001278601.1 TNF-α-R CAAATCGGCTGACGGTGTGG Real-time PCR primer, reverse IL6-F TGGGACTGATGCTGGTGACA Real-time PCR primer, forward NM_031168.2 IL6-R ACAGGTCTGTTGGGAGTGGT Real-time PCR primer, reverse ZO-2-F CCACCTCGCACGCATCACAG Real-time PCR primer, forward XM_017318137.1 ZO-2-R TGGTCCTTCACCTCTGAGCACTAC Real-time PCR primer, reverse Claudin-1-F TCGGCTCCATCGTCAGCACTG Real-time PCR primer, forward NM_016674.4 Claudin-1-R AGATGGCCTGAGCGGTCACG Real-time PCR primer, reverse IFN-γ-F GGCTCTGGAGGCTGGAGGAAG Real-time PCR primer, forward NM_008337.4 IFN-γ-R TGATAGGCGGTGAGGCTACAAGG Real-time PCR primer, reverse