绵羊SMAD1基因组织表达及其多态性与产羔数关联分析
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田志龙,汤继顺,孙庆,王玉琴,张效生,张金龙,储明星
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Tissue Expression and Polymorphism of Sheep SMAD1 Gene and Their Association with Litter Size
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TIAN ZhiLong,TANG JiShun,SUN Qing,WANG YuQin,ZHANG XiaoSheng,ZHANG JinLong,CHU MingXing
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表1 绵羊SMAD1基因的引物信息
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Table 1 Primer information of SMAD1 gene in sheep
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引物及探针名称 Primer names | 引物序列 Primer sequences | 退火温度 Annealing temperature (℃) | 扩增片段(bp) Product size | 用途 Usage | SMAD1 | F: TGGTTCCAAGACACAGCGAAT R: GGTGTATCTGCTGGCATCTGA | 60 | 253 | 半定量RT-PCR semi-quantitative RT-PCR | SMAD1Q | F: CCCGAGTGGGTGTAGTTT R: TCCTGGCGGTGGTATTCT | 60 | 162 | 荧光定量PCR qPCR | GAPDH | F: TGACGCTCCCATGTTTGTGA R: TCATAAGTCCCTCCACGATGC | 60 | 149 | 半定量RT-PCR 和荧光定量PCR semi-quantitative RT-PCR and qPCR | FecB-Taqman | F: CCAGCTGGTTCCGAGAGACA R: CTTATACTCACCCAAGATGTTTTCATG | 60 | 73 | FecB-Taqman分型 FecB-Taqman genotyping | FecB-Taqman P-G FecB-Taqman P-A | AAATATATCGGACGGTGTT-MGB AAATATATCAGACGGTGTTG-MGB | 60 |
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