绵羊SMAD1基因组织表达及其多态性与产羔数关联分析
田志龙,汤继顺,孙庆,王玉琴,张效生,张金龙,储明星

Tissue Expression and Polymorphism of Sheep SMAD1 Gene and Their Association with Litter Size
TIAN ZhiLong,TANG JiShun,SUN Qing,WANG YuQin,ZHANG XiaoSheng,ZHANG JinLong,CHU MingXing
表1 绵羊SMAD1基因的引物信息
Table 1 Primer information of SMAD1 gene in sheep
引物及探针名称
Primer names
引物序列
Primer sequences
退火温度
Annealing temperature (℃)
扩增片段(bp)
Product size
用途
Usage
SMAD1 F: TGGTTCCAAGACACAGCGAAT
R: GGTGTATCTGCTGGCATCTGA
60 253 半定量RT-PCR semi-quantitative RT-PCR
SMAD1Q F: CCCGAGTGGGTGTAGTTT
R: TCCTGGCGGTGGTATTCT
60 162 荧光定量PCR qPCR
GAPDH F: TGACGCTCCCATGTTTGTGA
R: TCATAAGTCCCTCCACGATGC
60 149 半定量RT-PCR
和荧光定量PCR semi-quantitative RT-PCR and qPCR
FecB-Taqman F: CCAGCTGGTTCCGAGAGACA
R: CTTATACTCACCCAAGATGTTTTCATG
60 73 FecB-Taqman分型
FecB-Taqman genotyping
FecB-Taqman P-G
FecB-Taqman P-A
AAATATATCGGACGGTGTT-MGB
AAATATATCAGACGGTGTTG-MGB
60