绵羊SMAD1基因组织表达及其多态性与产羔数关联分析

田志龙,汤继顺,孙庆,王玉琴,张效生,张金龙,储明星

Tissue Expression and Polymorphism of Sheep SMAD1 Gene and Their Association with Litter Size

TIAN ZhiLong,TANG JiShun,SUN Qing,WANG YuQin,ZHANG XiaoSheng,ZHANG JinLong,CHU MingXing

表1 绵羊SMAD1基因的引物信息

Table 1 Primer information of SMAD1 gene in sheep

引物及探针名称 Primer names 引物序列 Primer sequences 退火温度 Annealing temperature (℃) 扩增片段(bp) Product size 用途 Usage SMAD1 F: TGGTTCCAAGACACAGCGAAT R: GGTGTATCTGCTGGCATCTGA 60 253 半定量RT-PCR semi-quantitative RT-PCR SMAD1Q F: CCCGAGTGGGTGTAGTTT R: TCCTGGCGGTGGTATTCT 60 162 荧光定量PCR qPCR GAPDH F: TGACGCTCCCATGTTTGTGA R: TCATAAGTCCCTCCACGATGC 60 149 半定量RT-PCR 和荧光定量PCR semi-quantitative RT-PCR and qPCR FecB-Taqman F: CCAGCTGGTTCCGAGAGACA R: CTTATACTCACCCAAGATGTTTTCATG 60 73 FecB-Taqman分型 FecB-Taqman genotyping FecB-Taqman P-G FecB-Taqman P-A AAATATATCGGACGGTGTT-MGB AAATATATCAGACGGTGTTG-MGB 60