MT和FGF5调控辽宁绒山羊绒毛生长相关LncRNA的筛选及鉴定

金梅,张丽娟,曹倩,郭鑫英

The Screening and Identification of LncRNA Related to Villus Growth in Liaoning Cashmere Goats by MT and FGF5

JIN MEI,ZHANG LIJUAN,CAO QIAN,GUO XinYing

表1 RT-PCR引物序列

Table 1 RT-PCR Primer sequence

基因 Gene 引物 Primer 引物序列（5'→3'） Primer sequence(5'→3') 产物 Products β-actin β-actin -F β-actin -R GATGGCTACTGCTGCGTCG GGCATACAGGTCCTTTCGG 208bp LncRNA XLOC_005914 LncRNA XLOC_005914 -F LncRNA XLOC_005914-R CAGACCTTGCCACACCGA CAGTCCCGAACAAAATCAGC 143bp LncRNA XLOC_018763 LncRNA XLOC_018763 -F LncRNA XLOC_018763-R CAGCAGAGAAAGGAGAAAATGG GTGATGTAGGTGAGAGGCGAG 299bp LncRNA XLOC_011424 LncRNA XLOC_011424 -F LncRNA XLOC_011424-R AAAAGCACATTAGAGGCTGGAG GCAAGCAAATTCATGGCACA 116 bp LncRNA XLOC_009522 LncRNA XLOC_018763 -F LncRNA XLOC_018763-R GGGCGTGACATTTTCAGATT TCAGGGGACCTTTGGACTTG 289 bp LncRNA XLOC_009063 LncRNA XLOC_009063-F LncRNA XLOC_009063-R GCATCATTTCTTCCGCATTC TGGGTTACCATTTCCTTCTCC 220 bp LncRNA XLOC_011157 LncRNA XLOC_011157-F LncRNA XLOC_011157-R CTTTGGAAATGGTTCTGTGCTG GGAAGTAGGAGATGCTGGGTAA 140 bp