飞蝗内表皮结构糖蛋白基因LmAbd-2的表达与功能分析
贾盼1,2,张晶1,2,杨洋1,2,刘卫敏1,张建珍1,赵小明1(
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Expression and Function Analysis of Endocuticle Structural Glycoprotein Gene LmAbd-2 in Locusta migratoria
JIA Pan1,2,ZHANG Jing1,2,YANG Yang1,2,LIU WeiMin1,ZHANG JianZhen1,ZHAO XiaoMing1()
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图4. LmAbd-2 对飞蝗表皮结构的影响
A：若虫期连续注射dsLmAbd-2蜕皮至成虫72 h后LmAbd-2的相对表达量 The relative expression of LmAbd-2 determined by RT-qPCR after injected with dsLmAbd-2 72 h。B：注射dsRNA后,蜕皮至成虫72 h的表皮显微结构 After injection of dsRNA, microstructure and magnification of cuticle by H&E staining;红色为细胞质及细胞外基质,蓝色为细胞核 Cytoplasm and extracellular matrix are stained red with eosin, nuclei are stained dark blue with hematoxylin (Scale bars, 20 μm),Cut：表皮Cuticle;Ec：表皮细胞Epidermal cell。C：注射dsRNA后,蜕皮至成虫72 h的表皮超微结构观察 Ultrastructure observation of adult cuticle at 72 h after injected with dsRNA。Epi：上表皮Epicuticle;Exo：外表皮Exocuticle;Endo：内表皮Endocuticle
Fig. 4. Effect of LmAbd-2 on the formation of cuticle by H&E staining and TEM after injection of dsRNA
