飞蝗内表皮结构糖蛋白基因LmAbd-2的表达与功能分析
|
|
贾盼,张晶,杨洋,刘卫敏,张建珍,赵小明
|
Expression and Function Analysis of Endocuticle Structural Glycoprotein Gene LmAbd-2 in Locusta migratoria
|
|
JIA Pan,ZHANG Jing,YANG Yang,LIU WeiMin,ZHANG JianZhen,ZHAO XiaoMing
|
|
表2 本研究中所用引物
|
Table 2 Primer sequences used in this study
|
|
| 基因 Gene | 引物序列Primer sequence (5′-3′) | 用途 Application | 产物长度 Product length (bp) | | LmAbd-2 | F:taatacgactcactatagggATGCAGCTGTTGGTATGCCT | dsLmAbd-2合成
dsLmAbd-2 synthesis | 196 | | R:taatacgactcactatagggGCTCCTGGGCGGCGATGCCG | | GFP | F:taatacgactcactatagggTGGAGAGGGTGAAGG | dsGFP 合成
dsGFP synthesis | 571 | | R:taatacgactcactatagggGGGCAGATTGTGTGGAC | | LmAbd-2 | F:GTGTTTGACCCTGCTGGTG | RT-qPCR | 106 | | R:CCGTTGCCGTTTCGTATT | | β-actin | F:CGAAGCACAGTCAAAGAGAGGTA | 156 | | R:GCTTCAGTCAAGAGAACAGGATG |
|
|
|