柑橘溃疡病相关基因CsPGIP的克隆与表达
胡安华,祁静静,张庆雯,陈善春,邹修平,许兰珍,彭爱红,雷天刚,姚利晓,龙琴,何永睿,李强

Cloning and Expression Analysis of the Citrus Bacterial Canker-Related Gene CsPGIP in Citrus
HU AnHua,QI JingJing,ZHANG QingWen,CHEN ShanChun,ZOU XiuPing,XU LanZhen,PENG AiHong,LEI TianGang,YAO LiXiao,LONG Qin,HE YongRui,LI Qiang
表1 本研究使用的引物
Table 1 The primers used in this study
引物名称Primer name 引物序列Primer sequence (5′-3′) 酶切位点Enzyme site
OE-CsPGIP-f CGGGATCCATGAGCAACACGTCACTGTTGTCT BamHI
OE-CsPGIP-r CGGAATTCTCACTTGCAGCTTTCGAGGGGCGC EcoRI
SCL-CsPGIP-f GGGGTACCATGAGCAACACGT CACTGTTGT KpnI
SCL-CsPGIP-r TCCCCCGGGCTTGCAGCTTTCG AGGGGCGCG SmaI
qPCR-CsPGIP-f AGAAGCTTGGCGCTCTTCAT N/A
qPCR-CsPGIP-r TCGCCTTCAAGCTTGTTCCT N/A
qPCR-Actin-f CATCCCTCAGCACCTTCC N/A
qPCR-Actin-r CCAACCTTAGCACTTCTCC N/A
OE-f (35S) AGTAAGGATCGAT CCCACAAAGT N/A
OE-r (CsPGIP) TTTTTGAAGAGTAGTGAAGCTGCA N/A