柑橘溃疡病相关基因CsPGIP的克隆与表达

胡安华,祁静静,张庆雯,陈善春,邹修平,许兰珍,彭爱红,雷天刚,姚利晓,龙琴,何永睿,李强

Cloning and Expression Analysis of the Citrus Bacterial Canker-Related Gene CsPGIP in Citrus

HU AnHua,QI JingJing,ZHANG QingWen,CHEN ShanChun,ZOU XiuPing,XU LanZhen,PENG AiHong,LEI TianGang,YAO LiXiao,LONG Qin,HE YongRui,LI Qiang

表1 本研究使用的引物

Table 1 The primers used in this study

引物名称Primer name 引物序列Primer sequence (5′-3′) 酶切位点Enzyme site OE-CsPGIP-f CGGGATCCATGAGCAACACGTCACTGTTGTCT BamHI OE-CsPGIP-r CGGAATTCTCACTTGCAGCTTTCGAGGGGCGC EcoRI SCL-CsPGIP-f GGGGTACCATGAGCAACACGT CACTGTTGT KpnI SCL-CsPGIP-r TCCCCCGGGCTTGCAGCTTTCG AGGGGCGCG SmaI qPCR-CsPGIP-f AGAAGCTTGGCGCTCTTCAT N/A qPCR-CsPGIP-r TCGCCTTCAAGCTTGTTCCT N/A qPCR-Actin-f CATCCCTCAGCACCTTCC N/A qPCR-Actin-r CCAACCTTAGCACTTCTCC N/A OE-f (35S) AGTAAGGATCGAT CCCACAAAGT N/A OE-r (CsPGIP) TTTTTGAAGAGTAGTGAAGCTGCA N/A