大载体转染猪胎儿成纤维细胞的电转条件优化

钟翠丽,李国玲,王豪强,莫健新,全绒,张献伟,李紫聪,吴珍芳,顾婷,蔡更元

Optimizing the Electroporation Condition of Porcine Fetal Fibroblasts for Large Plasmid

ZHONG CuiLi,LI GuoLing,WANG HaoQiang,MO JianXin,QUAN Rong,ZHANG XianWei,LI ZiCong,WU ZhenFang,GU Ting,CAI GengYuan

图4 不同质粒拓扑结构的转染效率 A：荧光显微镜观察转染不同拓扑结构质粒的细胞;B：比较超螺旋和线性化质粒的转染效率。BTX：ECM? 830的最佳电转参数（脉冲电压300 V,脉冲长度1 ms,脉冲次数3次）;NEPA-4：NEPA 21的最佳电转参数（脉冲电压200 V,脉冲长度3 ms,脉冲间隔50 ms,脉冲次数3次）; LONZA-U：Nucleofector? 2b的最佳电转参数（U-023）;Sp：超螺旋质粒;Lp：线性化质粒

Fig. 4 The transfection efficiency of plasmid with different topological structures A: Cells transfected with the different topological structures of plasmid were observed by fluorescence microscopy; B: Comparison of transfection efficiency between supercoiled plasmid and linearized plasmid. BTX: The better parameter of ECM? 830(voltage 200 V, continuous 3 ms and 3 times); NEPA-4: The better parameter of NEPA 21(voltage 200 V, continuous 3 ms, interval 50 ms and 3 times); LONZA-U: The better parameter of Nucleofector? 2b(U-023); Sp: Supercoiled plasmid; Lp: Linearized plasmid.*: P<0.05; **: P<0.01