大载体转染猪胎儿成纤维细胞的电转条件优化

钟翠丽,李国玲,王豪强,莫健新,全绒,张献伟,李紫聪,吴珍芳,顾婷,蔡更元

Optimizing the Electroporation Condition of Porcine Fetal Fibroblasts for Large Plasmid

ZHONG CuiLi,LI GuoLing,WANG HaoQiang,MO JianXin,QUAN Rong,ZHANG XianWei,LI ZiCong,WU ZhenFang,GU Ting,CAI GengYuan

图2 不同电转参数条件下的转染效率 A：4种NEPA21电转参数的转染效率比较,NEPA-1~NEPA-4的电转参数见表2;B：2种Nucleofector? 2b电转参数的转染效率比较,LONZA-A：A-033;LONZA-U：U-023

Fig. 2 The transfection efficiency of different parameters A: Comparison of transfection efficiency between 4 kinds of NEPA21 electroporation parameters which are shown in Table 2; B: Comparison of transfection efficiency between two kinds of Nucleofector? 2b electroporation parameters, LONZA-A: A-033; LONZA-U: U-023 o