大载体转染猪胎儿成纤维细胞的电转条件优化

钟翠丽,李国玲,王豪强,莫健新,全绒,张献伟,李紫聪,吴珍芳,顾婷,蔡更元

Optimizing the Electroporation Condition of Porcine Fetal Fibroblasts for Large Plasmid

ZHONG CuiLi,LI GuoLing,WANG HaoQiang,MO JianXin,QUAN Rong,ZHANG XianWei,LI ZiCong,WU ZhenFang,GU Ting,CAI GengYuan

表2 优化Nucleofector? 2b电转参数的试验分组

Table 2 Grouping of optimizing the parameters of Nucleofector? 2b

组别 Group 电转参数 Parameters 电转预混液 Transfection mixture 处理组1 Group 1 LONZA-A1) 100 μL Nucleofector? 2b电转液3)+8 μg质粒 100 μL the transfection mixture of Nucleofector? 2b and 8 μg plasmid 处理组2 Group 2 LONZA-U2) 空白组Blank group 不电转Non transfection 100 μL Nucleofector? 2b电转液Only 100 μL the transfection mixture of Nucleofector? 2b