大载体转染猪胎儿成纤维细胞的电转条件优化
钟翠丽,李国玲,王豪强,莫健新,全绒,张献伟,李紫聪,吴珍芳,顾婷,蔡更元

Optimizing the Electroporation Condition of Porcine Fetal Fibroblasts for Large Plasmid
ZHONG CuiLi,LI GuoLing,WANG HaoQiang,MO JianXin,QUAN Rong,ZHANG XianWei,LI ZiCong,WU ZhenFang,GU Ting,CAI GengYuan
表2 优化Nucleofector? 2b电转参数的试验分组
Table 2 Grouping of optimizing the parameters of Nucleofector? 2b
组别
Group
电转参数
Parameters
电转预混液
Transfection mixture
处理组1 Group 1 LONZA-A1) 100 μL Nucleofector? 2b电转液3)+8 μg质粒
100 μL the transfection mixture of Nucleofector? 2b and 8 μg plasmid
处理组2 Group 2 LONZA-U2)
空白组Blank group 不电转Non transfection 100 μL Nucleofector? 2b电转液Only 100 μL the transfection mixture of Nucleofector? 2b