大载体转染猪胎儿成纤维细胞的电转条件优化

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Optimizing the Electroporation Condition of Porcine Fetal Fibroblasts for Large Plasmid

ZHONG CuiLi,LI GuoLing,WANG HaoQiang,MO JianXin,QUAN Rong,ZHANG XianWei,LI ZiCong,WU ZhenFang,GU Ting,CAI GengYuan

表1 NEPA21的穿孔脉冲参数

Table 1 Poring pulse of NEPA21

编号 Number 脉冲电压 Voltage (V) 脉冲长度 Length (ms) 脉冲间隔 Continuous (ms) 脉冲次数 Times 衰减幅度 Voltage attenuation range (%) 电极 Electrode NEPA-1 150 5 50 2 10 + NEPA-2 200 3 50 3 10 + NEPA-3 275 1.5 50 2 10 + NEPA-4 300 1 50 3 10 +