大载体转染猪胎儿成纤维细胞的电转条件优化
钟翠丽,李国玲,王豪强,莫健新,全绒,张献伟,李紫聪,吴珍芳,顾婷,蔡更元

Optimizing the Electroporation Condition of Porcine Fetal Fibroblasts for Large Plasmid
ZHONG CuiLi,LI GuoLing,WANG HaoQiang,MO JianXin,QUAN Rong,ZHANG XianWei,LI ZiCong,WU ZhenFang,GU Ting,CAI GengYuan
表1 NEPA21的穿孔脉冲参数
Table 1 Poring pulse of NEPA21
编号
Number
脉冲电压
Voltage (V)
脉冲长度
Length (ms)
脉冲间隔
Continuous (ms)
脉冲次数
Times
衰减幅度
Voltage attenuation range (%)
电极
Electrode
NEPA-1 150 5 50 2 10 +
NEPA-2 200 3 50 3 10 +
NEPA-3 275 1.5 50 2 10 +
NEPA-4 300 1 50 3 10 +