干酪乳杆菌CRISPR基因座分析

杨兰,杨洋,李伟勋,ObaroakpoJOY,逄晓阳,吕加平

CRISPR Locus Analysis of Lactobacillus casei

YANG Lan,YANG Yang,LI WeiXun,OBAROAKPO JOY,PANG XiaoYang,LÜ JiaPing

表2 干酪乳杆菌spacer对应的原间隔区序列特点

Table 2 Sequence characteristics of the original spacer corresponding to L. casei spacer

间隔 Spacer 原间隔区 Original interval 开放阅读框 Open reading box 匹配性 Matching BD-Ⅱ间隔区21 BD-Ⅱspacer21 乳杆菌质粒pREN Lactobacillus rennini plasmid pREN 假定蛋白质 Assuming protein 30/30 BL23间隔区21 BL23 spacer21 乳杆菌质粒pREN Lactobacillus rennini plasmid pREN 假定蛋白质 Assuming protein 30/30 LC2W 间隔区21 LC2W spacer21 乳杆菌质粒pREN Lactobacillus rennini plasmid pREN 假定蛋白质 Assuming protein 30/30 W56 间隔区21 W56 spacer21 乳杆菌质粒pREN Lactobacillus rennini plasmid pREN 假定蛋白质 Assuming protein 30/30 ZHANG间隔区14 ZHANG spacer14 乳酸乳球菌UL8质粒pUL8C Lactococcus lactis subsp. lactis strain UL8 plasmid pUL8C ─ 29/30 乳酸乳球菌乳脂亚种UC109 质粒pUC109F Lactococcus lactis subsp. cremoris strain UC109 plasmid pUC109F ─ 29/30 乳酸乳球菌C10 质粒pC10A Lactococcus lactis subsp. lactis strain C10 plasmid pC10A ─ 29/30 乳酸乳球菌KLDS 4.0325 质粒 unnamed2 Lactococcus lactis subsp. lactis KLDS 4.0325 plasmid unnamed2 ─ 29/30 乳酸乳球菌质粒pCL2.1 Lactococcus lactis plasmid pCL2.1 ─ 29/30 ZHANG间隔区15 ZHANG spacer15 乳酸杆菌TMW 1.1992 质粒 pL11992-8 Lactobacillus backii strain TMW 1.1992 plasmid pL11992-8 ─ 29/30 副干酪乳杆菌质粒pLP5403 Lactobacillus paracasei plasmid pLP5403 假定蛋白质 Assuming protein 29/30 卡氏双球菌ATCC BAA-344 质粒 pPECL-1 Pediococcus claussenii ATCC BAA-344 plasmid pPECL-1 ─ 29/30 植物乳杆菌质粒pXY3 Lactobacillus plantarum plasmid pXY3 ORF4 29/30 短乳酸杆菌质粒pLB925A01 Lactobacillus brevis plasmid pLB925A01 ─ 29/30 乳明串珠球菌质粒pCI411 Leuconostoc lactis plasmid pCI411 ─ 29/30 植物乳杆菌MF1298质粒19 Lactobacillus plantarum strain MF1298 plasmid unnamed19 ─ 28/30 戊糖片球菌SRCM100892质粒pPC892-5 Pediococcus pentosaceus strain SRCM100892 plasmid pPC892-5 ─ 27/30