越橘VcNAC072克隆及其促进花青素积累的功能分析

宋杨,刘红弟,王海波,张红军,刘凤之

Molecular Cloning and Functional Characterization of VcNAC072 Reveals Its Involvement in Anthocyanin Accumulation in Blueberry

SONG Yang,LIU HongDi,WANG HaiBo,ZHANG HongJun,LIU FengZhi

表1 本研究中使用的引物

Table 1 Primers used in this study

用途 Use 引物名称Primer name 序列（5′-3′）Primer sequence (5′-3′) 构建pRI101-VcNAC072表达载体 The construction of pRI101-VcNAC072 expression vector 构建pGADT7-VcNAC072表达载体 The construction of pGADT7-VcNAC072 expression vector 构建pAbAi-AtPAP1pro表达载体 The construction of pAbAi-AtPAP1pro expression vector 构建AtPAP1pro-GUS表达载体 The construction of AtPAP1pro-GUS expression vector 实时荧光定量qRT-PCR Real time fluorescent quantitative PCR VcNAC072(pRI101)-F VcNAC072(pRI101)-R VcNAC072( pGADT7)-F VcNAC072( pGADT7)-R AtPAP1pro(pAbAi)-F AtPAP1pro(pAbAi)-R AtPAP1pro(GUS)-F AtPAP1pro(GUS)-R VcNAC072-(RT-PCR)-F VcNAC072-(RT-PCR)-R VcGAPDH-(RT-PCR)-F VcGAPDH-(RT-PCR)-R AtUBQ10-(RT-PCR)-F AtUBQ10-(RT-PCR)-R AtPAP1-(RT-PCR)-F AtPAP1-(RT-PCR)-R AtDFR-(RT-PCR)-F AtDFR-(RT-PCR)-R AtANS-(RT-PCR)-F AtANS-(RT-PCR)-R GGAATTCCATATGATGGGAGTTCAGGAGTCCG CGCGGATCCTCACTGCCGAAACCCGAATCC GGAATTCCATATGATGGGAGTTCAGGAGTCCG CGCGGATCCTCACTGCCGAAACCCGAATCC CGAGCTCCTAATACATAAAATGTGGATATC ACGCGTCGACGGAACAAAGATAGATACGTAA CGGGGTACCCTAATACATAAAATGTGGATATC CATGCCATGGGGAACAAAGATAGATACGTAA GATGAGGTACAATGACATG GAGTTCTGCTGAAACAAC GGTTATCAATGATAGGTTTGGCA CAGTCCTTGCTTGATGGACC CGTTAAGACGTTGACTGGGAAAACT GCTTTCACGTTATCAATGGTGTCA GCTCTGATGAAGTCGATCTTC CTACCTCTTGGCTTTCCTCT GTCGGTCCATTCATCACAAC TGAGCGTTGCATAAGTCGTC TCAAGAAAGCCGGAGAAGAG TTGTCCACTCGCGTTGTTAG