褐飞虱一个新的海藻糖合成酶基因的特性、 发育表达及RNAi效果分析

唐斌,沈祺达,曾伯平,肖仲久,邱玲玉,潘碧莹,李昆,张道伟

Characteristics, Developmental Expression and RNAi Effect Analysis of a Novel Trehalose-6-Phosphate Synthase Gene in Nilaparvata lugens

TANG Bin,SHEN QiDa,ZENG BoPing,XIAO ZhongJiu,QIU LingYu,PAN BiYing,LI Kun,ZHANG DaoWei

表1 荧光实时定量PCR引物和TPS3及GFP的dsRNA引物序列

Table 1 Primers used for qRT-PCR and primers of dsTPS3 and dsGFP[31]

基因名称 Primer name 正向引物 Forward primer (5′-3′) 反向引物 Reverse primer (5′-3′) QNl18S QNlTPS1 QNlTPS2 QNlTPS3 CGCTACTACCGATTGAA AAGACTGAGGCGAATGGT AGAGTGGACCGCAACAACA GTGATGCGTCGGTGGCTAT GGAAACCTTGTTACGACTT AAGGTGGAAATGGAATGTG TCAACGCCGAGAATGACTT CCGTTCATCATTGGGCATAGT dsNlTPS3 GAGTCTGACCTGATAGCCTTTA ATCGGAGTCCATTTAGTTGT dsNlTPS3-T7 GGATCCTAATACGACTCACTATAGGGAGTCTGACCTGATAGCCTTTA GGATCCTAATACGACTCACTATAGGATCGGAGTCCATTTAGTTGT dsNlGFP AAGGGCGAGGAGCTGTTCACCG CAGCAGGACCATGTGATCGCGC dsNlGFP-T7 GGATCCTAATACGACTCACTATAGGAAGGGCGAGGAGCTGTTCACCG GGATCCTAATACGACTCACTATAGGCAGCAGGACCATGTGATCGCGC